Composite

Part:BBa_K1884008:Design

Designed by: Changxing Hu   Group: iGEM16_SZU-China   (2016-10-11)


PSAD Promoter+GalAD-CRY2+UAS+PsbA+PSAD Terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3123
    Illegal NotI site found at 3221
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1560
    Illegal BglII site found at 2019
    Illegal BamHI site found at 2498
    Illegal XhoI site found at 3052
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1444
    Illegal AgeI site found at 2173
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1796
    Illegal BsaI.rc site found at 1205
    Illegal SapI.rc site found at 1313
    Illegal SapI.rc site found at 3384


Design Notes

Source

PSAD promoter and PSAD terminator are gained from the old parts in iGEM, AD-CRY2 and UAS are gained from Shenzhen Engineering Laboratory of Marine Algal Biotechnology.


References